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Animal and in vitro experiments have further verified its barrier repair effect: Butyrate can promote proliferation and mucin secretion of intestinal epithelial cells (IPECs, Caco-2, HT-29), enhance the expression of tight-junction proteins (ZO-1, Claudin-1, Claudin-2), and maintain colonic mucosal integrity (7682)

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Most formulations available commercially are marketed as research compounds, and regulatory agencies have expressed concerns about the safety and quality control of unregulated peptide products